Scale ADT assay from 0 to 1 per feature — ScaleADT • pochi

Scale ADT assay from 0 to 1 per feature

Usage

ScaleADT(
  object,
  assay = "ADT",
  layer = "data",
  split.by = NULL,
  low.p = 0.001,
  high.p = 0.999,
  scale.to.one = FALSE,
  new.assay = "sADT"
)

Arguments

object: Seurat object
assay: Which assay, typically ADT
layer: Which layer in the assay, typically data after CLR
split.by: If not NULL, which metadata feature to split by before scaling.
low.p: Which percentile to set as 0
high.p: Which percentile to set as 1
new.assay: Name of new assay to save values to

Value

Returns a Seurat object with scaled values in the data slot of the specified assay

Details

Scales the expression values for each feature in the specified assay from 0 to 1, with an optional split.by argument